whats the difference between RNA extraction(with trisol)when we want to measure long non coding RNA and when we want to measure gene expression?

whats the difference between RNA extraction(with trisol)when we want to measure long non coding RNA and when we want to measure gene expression?

07 September 2020 7,431 5 View

Has anyone had problem with single cell whole genome amplification?

Hello, I am experiencing problem with single cell whole genome amplification from 10-12 cells only 3 or 4 amplifies and I have a smear on gel electrophoresis, Can anyone help me? Best Regards

12 February 2018 3,212 5 View

Modeling Nucleate Boiling Using ANSYS FLUENT

20 June 2016 5,466 6 View

How should I use patch function for defining liquid-vapor interface?

Hi. I am working on a simulation of evaporation in a capillary tube.I am simulating evaporation of a water droplet sitting at the end of a capillary tube inside a solid body subjected to heat...

16 June 2016 508 16 View

Can I do with COMSOL simulation of intense laser plasma interaction and I want to expose this laser-produced plasma in an elecric field?

I need COMSOL code for simulation of intense laser plasma interaction and I want to expose this laser-produced plasma in an electric field to see double layering and then seed this laser-produced...

03 April 2016 1,597 2 View

How can I remove the staining from the background in IHC?

I am doing IHC using an ABC staining kit (centacruz), concentration of primary and secondary antibody is optimized to detect the desired protein, incubate DAB 30 min, counterstain 5 second, but...

08 March 2013 3,561 2 View

What is the best way to increase the concentration of proteins?

I extracted total proteins from tissue to do western blot. Some of my samples are in low concentration. Since I need to run equal proteins for SDS page, I want to increase the concentration of...

08 February 2013 1,857 25 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View

How to obtain parameters to run a simulation for benzene using CHARMM forcefield in GROMACS?

I have been reading this paper Soft Matter, 2017, 13, 1904 and in this paper, they simulate benzene in GROMACS using CHARMM. They mention that they performed simulations of benzene using the...

15 February 2021 5,561 3 View

Can I use the CHARMM GUI input files for Gromacs in HPC?

Good day everyone! I am a Biochemistry undergraduate in the Philippines and to be very honest, we have no background in bioinformatics since we really focused on wet lab techniques + theory. Due...

15 February 2021 3,422 4 View

Charmm/GROMACS Residue 'ZN' not found in residue topology database?

I am trying to do some MD simulations of a protein with zinc ions. I am trying to use a charmm forcefield (I have tried 2017 and 2021) and Gromacs. I get an error when doing pdb2gmx Residue 'ZN'...

12 February 2021 9,367 2 View

Defining effective diffusivity in porous zone and non-porous zone?

Hi all, I am working on methanol steam reforming using packed bed for hydrogen generation. I am using FLUENT software for CFD simulation. In reference paper, authors mentioned that effective mass...

10 February 2021 6,104 1 View

Online Shopping Security Survey?

I would appreciate if you answer my DBA research questionnaire. Thank you all in...

10 February 2021 1,990 3 View

Anyone use GROMACS' pdb2gmx for Adenosine monophosphate (AMP) to use with CHARMM force field?

I am trying to use the following command in GROMACS to generate a CHARMM topology file: gmx pdb2gmx -ff charmm27 -f amp.pdb -o amp.gro -p amp.top The atom list in my pdb file (amp.pdb)...

01 February 2021 7,769 6 View

Is there any difference between "SEP" as a non standard residue and phohophorylated serine?

I am trying to run a molecular dynamics simulation for a ligand that contains a "SEP" residue. also, I am preparing my files using Charmm-GUI using charmm36 ff, but it says that it will rename my...

31 January 2021 6,321 1 View

Does AMBER or CHARMM force field parameters have coarsed grained side chain parameters?

I need to coarse grain the side chain with a single atom. However, what force field parameters do I have to use. Does AMBER or CHARMM have any parameters and partial charges for coarse-grained...

31 January 2021 7,091 3 View

Can't run my IPMSM drive system with DC-supply higher than 125 V. Have anyone had similar problem?

Hey, I try to run my 4 pole IPMSM with PWSVM and use a typical controller with PI-controller with a current loop and speed loop. When I run my system with DC-supply 100 V with a steady-state speed...

28 January 2021 7,354 8 View