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Questions related from Adron Ung
Some (but not all) DNA polymerases such as Klenow fragment, Taq, and Phi 29 DNA polymerase can catalyze strand displacement synthesis. This is evidenced by opening of molecular beacons and Loop...
12 December 2023 3,784 1 View
If an active site mutant knocks out product formation at all excessive concentrations of substrate and at all excessive concentrations enzyme, but substrate binding affinity via anisotropy shows...
17 October 2023 2,016 4 View
I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...
10 February 2021 5,401 5 View
Hey scientists, I am intending to observe the extension of a short 10-mer primer on a 16-mer template. I just use a certain DNA polymerase to extend the primer on a templating strand with a...
05 November 2020 6,839 8 View
Hey scientists, I am just wondering what functional groups can be added to a hydrophobic molecule to make it water soluble at basic pH? As a case study, let's just consider ethyl benzene as the...
15 October 2020 733 9 View
So I am trying to crystallize a Protein:DNA complex with DNA at at least 5 times excess at basic pH with a certain poleythene glycol molecular weight (PEG) and a mixture of soluble salts at low...
29 September 2020 8,239 4 View
Hello fellow scientists, I have some additional questions about the dissociation constant for a protein binding ligand. My main questions is if the protein concentration is much greater than the...
26 August 2020 2,993 8 View
Hey scientists, I am trying to drug a protein which is soluble between a pH of 5.0 to 9.0. My goal is to crystallize an amine drug with this protein and I know optimal conditions that crystallize...
20 June 2020 604 0 View
So I have some pH = {4, 7, 10} standard buffer solutions from Fisher Scientific. But they must have went bad or I must have contaminated them because when I try to calibrate my pH meter to 4 using...
14 June 2020 4,245 11 View
So I am a new PhD student but I have been doing good research since I have been an undergrad. I have scientific interest in a lot of things and firmly believe that "A change in perspective is...
10 November 2019 3,554 6 View
This is my understanding. If it is wrong, please correct me. So animals have an adaptive immune system where when they come into contact with a foreign substance they create antibodies to it and...
20 October 2018 516 11 View
Hello scientists, I wonder why it is that when I try to take a picture through a microscope with my camera holding my camera (my iPhone) steady as I am able to next to my microscope to take a...
20 April 2017 4,992 4 View
Hello scientists, When I think about genetic diseases like cystic fibrosis, sickle cell anemia, diabetes, and various forms of cancer I don't imagine that those can be easily treated by surgery...
11 March 2017 4,441 3 View
Hello, thank you for your help. So first of all I have a lot of .gjf files (Gaussian acceptable format) and I want to run Gaussian on all of them to get energy log files. I think that's a...
18 February 2017 8,972 5 View
Hello, so I have several Protein Data Bank (.pdb) files that have been parsed to only include two residues (Tryptophan with Glutamate, and Tryptophan with Aspartate) in a protein. I want to...
07 December 2016 1,086 2 View
So I basically have a general question, not for any compound in particular, just a standard protocol. If you know the balanced chemical reaction to produce a desired compound, but the ΔS's, ΔH's,...
03 November 2016 6,265 5 View
Hello, I noticed that there was an article about QwikMD which makes running the NAMD simulations easier, complex, and efficient. But I have a problem. I'm just doing the basic example where I have...
19 October 2016 9,853 3 View
Hello, My question may be simple. But I think others could benefit from having this skill, and I'm not quite sure how to separate these files once I get them. So I was looking through a molecular...
15 September 2016 847 5 View
I've been data-mining and I can't find anything lately along the lines of my questions. I've noticed that a lot of researchers like to do their molecular dynamics simulations with AMBER and then...
06 July 2016 660 4 View
I'm just totally amazed. I mean, I have found information about this parasite from reading research papers. But the CDC just doesn't have any internet accessible content about it ... just blows my...
17 April 2016 6,820 5 View
Okay, so I have a template program from Beginning Perl for Bioinformatics, example 4 - 4. However, it works for calculating the reverse complement of a DNA strand given a predetermined DNA...
06 April 2016 8,196 3 View
Are you aware of any FPLC vs HPLC comparison studies of a given protein, evaluated under virtually the same conditions to see the final stability or quantification of the protein assessed (and...
28 March 2016 4,092 8 View
Hello, I'm an undergraduate. First of all, I have a few questions about FPLC which are of proof of concept, not that I'm looking to detect a particular protein. I would like to know if a High...
13 March 2016 2,203 6 View
I do like Cell, Nature, & Science. I find the discoveries to be amazing. But the experiments can be difficult to understand and difficult to replicate without the state of the art scientific...
01 January 1970 6,651 13 View
Main question: What is the electric field strength of microalgae? Background: So I have been having some problems with fungi contaminating my chromera velia algae cell cultures. I haven't...
01 January 1970 7,213 1 View
Do you think scientists are more susceptible to concluding that a false positive is a real positive or that a false negative is a real negative? As I understand, A) A false positive is when a...
01 January 1970 8,359 8 View
So I'm new to Gibson Assembly. I have done restriction enzyme ligation before. As I understand, Gibson Assembly inserts a gene of interest into a the backbone of the vector primer by having the...
01 January 1970 9,327 9 View
