Hello, In order to speed up our protein production process, we would like to use an affinity purification TAG. Some well-known TAGs work very well (strepTag) but identifying a cGMP compliant column is a challenge, others like His TAG have cGMP compliant columns but are difficult to use due to the risk associated with nickel and imidazole. We have identified C-Tag. Do any of you have recommendations or other suggestions for us? Thank you
This tag looks a good choice given its success with malarial antigens. You will, of course, be subject to licensing by the owners of the IP.
Is "speed up" really the correct goal in drug production? I don't think so. You're also talking about putting your tag (or a residue after removal) into the patient. All this can be OK, if you document it well and have the safety data to back it up. But the less of it you do the better.
If you have a purification process that works without affinity tag, I think the best thing you can do for both your patients and for the regulator is to keep using it... I think "speed up" is the wrong goal that has very little value to anyone except your technician. And you solve that best by hiring a second technician.
- Badges
- Science method