Dominique Blanchard

3 Questions 9 Answers 0 Followers

Questions related from Dominique Blanchard

Antibody : loss of binding at high concentration (beyond saturation) Why?

Hi everyone, It is commonly observed that some antibodies presents a binding saturation curve that goes down at high concentration (at concentration beyond saturation). Does anybody have a good...

21 August 2020 937 5 View

What are the options for protein sequestration in the cytoplasm or for inducing degradation?

Hi, I'm looking for mutation (fusion with signal peptide) to sequester a protein within the cytoplasm or to induce it's degradation. I was thinking to something like CAAX, KDEL, or other motif....

25 August 2017 2,406 3 View

CGMP compliant protein affinity TAG

Hello, In order to speed up our protein production process, we would like to use an affinity purification TAG. Some well-known TAGs work very well (strepTag) but identifying a cGMP compliant...

01 January 1970 1,207 3 View