Hi everybody,
here i am with another question. I am working with adherent fish cells but i am now facing the problem, that the cells are detaching after a few days. After seeding, the cells attached fine, they strech and grow fine also. But for example today, on day 7, i find a lot of detached, round cells in suspension. They do not look like dead cells. In my experience, dead cells are more like round, dense, glowing dots (kind of like freshly seeded cells). But these cells i found today, the look like little balloons. Like a blown up cell with a thin skin floating around the media. Also on some attached cells you can see some arms starting to detach.
I really don´t know why they start to grow fine, and after some days they all just detach and don´t grow anymore. I change part of the media regularly so they are not starving.
I would be very grateful for your help!
Thank you so much in advance
Anika
Cell detachment can occur due to various factors, and troubleshooting the problem requires careful consideration of several potential causes. Here are some possibilities to consider:
You might need to perform controlled experiments, such as altering one factor at a time, to determine the exact cause of the detachment problem.
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