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Questions related from Anika Dreier
Hello fellow researchers! We are currently looking for a reliable source where we could get eggs from the sheepshead minnow to start a laboratory population. Does someone work with this species...
last month 8,437 0 View
Hello, i did a cell viability assay on a cell line after exposure to a test substance. The test worked fine and was valid in all criteria. The cells were stained with alamar blue and CFDA-AM as...
01 August 2025 9,198 1 View
Hi there, i did a little project on the side with a student in which i wanted to check, if my Fishcellline RTgillW1 can be tested at different pH Values. So i adjusted the pH value of my Medium...
04 October 2024 7,616 1 View
Hi, i need your insight on the following problem: I have issues with my Neutral Red viability assay. I have a very high variation in my cell free controls when i do the Neutral Red staining. I...
23 August 2024 307 0 View
Hi everybody, i need to pick your brains: i work with an adhesive fish cell line which forms a konfluent monolayer. I want to test an adsorbant substance, therefore i want to saturate the cell...
01 July 2024 9,428 0 View
Hello fellow researchers, i need some advise. I am trying to incubate some cells with a volatile substance and i am looking for ways to keep the substance from evaporating and keeping it in the...
07 March 2024 6,832 2 View
Hi everybody, ich have made an observation in my Fish gill cell culture. They are epithelial like cells and they grow nice and normal. But now in my passage 10 i did observe some formations which...
09 February 2024 2,781 0 View
Hi everybody, i am totally new to the programm Image J and i tried to use it to estimate my confluency in my cell culture. I found some descriptions but they do not fit for my picture at all......
26 September 2023 8,353 3 View
Hi, again i have a question about my fishcells. I thawed a new aliquot from a Cellbank according to protocol. I did wash them before seeding to get rid of the DMSO. I seeded about 1 Million cells...
20 September 2023 9,684 15 View
Hi everyone, i might have a dumb question... my fish cells keep detaching and dying after a few days of culture so no i am trying to find a solution to this problem. They grow at 19°C without...
05 September 2023 5,210 5 View
Hi everybody, here i am with another question. I am working with adherent fish cells but i am now facing the problem, that the cells are detaching after a few days. After seeding, the cells...
29 August 2023 2,364 1 View
Hey all my cell culture fellows, i need your help and experience... i am trying to establish a cell line in our lab. i bought a fish cell line and i am now trying to get it startet. But it is...
22 August 2023 1,744 4 View
So i am trying to establish a cell line in our lab, and i asked myself: If i have the feeling on day 2 or 3 of the initial cell culture, that my cell density is too low, what options do i have? Is...
03 August 2023 9,059 3 View
