Hi everybody, i need to pick your brains:
i work with an adhesive fish cell line which forms a konfluent monolayer. I want to test an adsorbant substance, therefore i want to saturate the cell culture plate with this substance. Problem is: i cant seed the cells before saturation, because then they would be exposed to the substance prior to my actual test. I cant seed them after saturation, because the substance might be toxic and kill the cells before i could do the actual test.
So now my idea was to seed the cells onto inserts in one 24well plate, saturate another plate and just transfer the insert into to saturatet plate to start the test. Would that be possible?
My test ends with a measurement of fluorescence of three dyes. Can i measure the fluorescence with the inlet still in the well? do i have to take the insert out to measure fluorescence in the supernatant? One of the dyes (Neutral Red) will be held inside the cells, is it possible to measure this one on an insert ( because it will not be at the bottom of the plate?).
I would be grateful for some ideas!
Thank you !