the product size is 191bp would it be a problem during conventional pcr?

I have designed a primer for Bacillus subtilis sfp gene. As I have mentioned the size is 191 bp. however I need some suggestions regarding PCR temperatures and cycle length. As the it is smaller...

09 January 2021 8,309 4 View

Can you help with the implementation of two different model of deep learning for stock market prediction?

I have stock price data(Open, high, low, close), I want to do prediction by combining two different models like CNN with others. Dataset will be same, what are the ways that I can use for...

04 January 2021 1,845 3 View

If we forget to add mercaptoethanol, can we correct the samples somehow?

Hi everyone, Can someone help me with a western blotting troubleshooting? So during sample prep, we boil the samples with 2-mercaptoethanol in Laemli buffer. However by mistake, some of the...

02 December 2020 6,859 3 View

How to isolate human cardiac stem cells? Which other cell types could be in this cell culture?

Hello, I followed a protocol for isolating human cardiac stem cells (Human cardiomyocyte progenitor cells differentiate into functional mature cardiomyocytes: an in vitro model for studying human...

18 November 2020 2,928 3 View

Please where can i buy pure Nitrogen gas in Nigeria?

I need just a very small quantity of pure Nitrogen gas for use in my locally designed anaerobic glove box. Any information would be of immerse help to my research work. Thanks

11 October 2017 2,853 6 View

How can I create the .nk files of materials in Silvaco atlas?

I'm a simulating a solar cell which includes the material GaAsP. The .nk file of GaAsP is not available in the sopra database..if i have the n,k data of this material can anyone tell me how can i...

21 March 2016 1,906 8 View

How do I upscale data from farm scale to village scale and to regional scale?

I am going to prepare a survey for farmers in East Africa. The data aquired I want to upscale to village scale and from there to regional scale. I am searching established methods for this...

14 December 2015 9,083 3 View

Does anybody know about an anti-mouse CD11d antibody that works in FACS?

Thanks in advance for your answers.

24 March 2014 659 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View