Problems in silver staining of gels?

Hi, I have tried silver staining many times to stain gels, but i dont get the results. After incubating the gels in silver nitrate, i do not get any results.I have changed my silver nitrate too,...

03 April 2018 2,711 4 View

Chemically induced Glioblastoma Animal Model?

Hi, Can anyone advise me about chemically induced GBM model? How valid they are in human GBM research? Thanks

09 October 2017 7,722 1 View

Is there any tool/software etc through which I can search proteins based on its molecular weight?

Hi all, I have got some differentially expressed bands (proteins) on my SDS-PAGE, having molecular weight 40 and 35 kDa. Now I want to find out which proteins are they. Is there any software/tool...

07 August 2017 9,398 0 View

Quantity of proteins from frozen cell lines and fresh culture?

Hi all, I am extracting proteins from cells frozen at -80C. Will it effect the quantity of proteins as compared to the quantity extracted from fresh cell cultures? Thankyou

07 August 2017 8,014 3 View

Bioinformatics tools for proteins?

Hi all, I need to apply some bioinformatics tools on some novel proteins and publish it. Kindly help.

06 July 2017 9,585 3 View

Can anybody check the review I have written for publication?

Hi, I have written a review entitled: ABBERANT GLYCOSYLATION OF CELL ADHESION MOLECULES (CAMs) IN PROGRESSION OF CANCER: HIGHLIGHTING GLIOMAS. Can anybody check it for me before i go for its...

05 June 2017 646 1 View

Lab groups working on proteomic analysis of Glioblastoma Multiforme??

02 March 2017 10,000 2 View

Lectin Probed Western Blotting?

I need a protocol for Lectin probed western blotting for the analysis of differentially expressed glycoproteins.

31 December 2016 135 1 View

Protein Extraction from U87 MG cell lines?

Hi, Previously, I had done protein extraction from tissue (mice brian). Now I need to extract proteins from cell lines (U87 MG). Can i follow the same protocol? If someone has a protocol or...

31 December 2016 6,575 3 View

What is the best method for Staining and Destaining the gels in SDS-PAGE?

Hi, Someone please explain me the exact solutions which can stain and destain the gels after SDS-PAGE.

31 December 2016 5,241 11 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View