Sumera Sumera

16 Questions 8 Answers 0 Followers

Questions related from Sumera Sumera

Problems in silver staining of gels?

Hi, I have tried silver staining many times to stain gels, but i dont get the results. After incubating the gels in silver nitrate, i do not get any results.I have changed my silver nitrate too,...

04 April 2018 2,792 4 View

Chemically induced Glioblastoma Animal Model?

Hi, Can anyone advise me about chemically induced GBM model? How valid they are in human GBM research? Thanks

10 October 2017 7,843 1 View

Is there any tool/software etc through which I can search proteins based on its molecular weight?

Hi all, I have got some differentially expressed bands (proteins) on my SDS-PAGE, having molecular weight 40 and 35 kDa. Now I want to find out which proteins are they. Is there any software/tool...

08 August 2017 9,446 0 View

Quantity of proteins from frozen cell lines and fresh culture?

Hi all, I am extracting proteins from cells frozen at -80C. Will it effect the quantity of proteins as compared to the quantity extracted from fresh cell cultures? Thankyou

08 August 2017 8,064 3 View

Bioinformatics tools for proteins?

Hi all, I need to apply some bioinformatics tools on some novel proteins and publish it. Kindly help.

07 July 2017 9,628 3 View

Can anybody check the review I have written for publication?

Hi, I have written a review entitled: ABBERANT GLYCOSYLATION OF CELL ADHESION MOLECULES (CAMs) IN PROGRESSION OF CANCER: HIGHLIGHTING GLIOMAS. Can anybody check it for me before i go for its...

06 June 2017 692 1 View

Lab groups working on proteomic analysis of Glioblastoma Multiforme??

Hi all, I have got a six months research fellowship. I am in search of some relevant labs to work in. My PhD project aims at the identification and characterization of novel altered proteins in...

03 March 2017 10,080 2 View

Lectin Probed Western Blotting?

I need a protocol for Lectin probed western blotting for the analysis of differentially expressed glycoproteins.

01 January 2017 182 1 View

What is the best method for Staining and Destaining the gels in SDS-PAGE?

Hi, Someone please explain me the exact solutions which can stain and destain the gels after SDS-PAGE.

01 January 2017 5,283 11 View

Protein Extraction from U87 MG cell lines?

Hi, Previously, I had done protein extraction from tissue (mice brian). Now I need to extract proteins from cell lines (U87 MG). Can i follow the same protocol? If someone has a protocol or...

01 January 2017 6,620 3 View

Cell lines as control against Glioblastoma cell lines (U87)?

Hi, I am working on Glioblastoma cell lines (U87) and looking for aberrantly expressed glycosylated proteins. which cell lines can I use as control? Can I use normal mice brain tissues as control?

12 December 2016 4,625 3 View

Are there potential hazards/serious adverse effects while dealing with brain tumor tissues in lab?

I am planning to work on human brain tumor tissues as part of my research. I need to know if certain potential hazards are associated with it?

10 October 2016 9,751 0 View

Can I use Lectins for Western Blot Detection of Glycoproteins?

Hi guys, Can i use Lectins instead of antibodies for the detection of Glycoproteins in Western Blotting?

08 August 2016 5,851 5 View

I need U87 Glioblastoma Cell Lines. Can any one provide me?

Hi Guys. I need U87 GBM cell lines. Can anyone help me.

08 August 2016 5,668 1 View

Protien Extraction Prortocol from cell lines?

Hi guys, I need a protein extraction protocol from cell lines. Regards

08 August 2016 1,286 1 View

Which monoclonal antibodies can I use for the enrichment of all the general determinants of glycoproteins?

I want to enrich all the Glycoproteins from my sample. No monoclonal antibodies have been identified that bind general determinants of glycosylated proteins, rather antibodies bind...

08 August 2016 5,878 2 View