I need a protocol for Lectin probed western blotting for the analysis of differentially expressed glycoproteins.
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Methods Mol Biol. 2014;1200:93-100. doi: 10.1007/978-1-4939-1292-6_8.
Lectin-probed western blot analysis.
Sato T1.
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Abstract
Lectin-probed western blot analysis, the so-called lectin blot analysis, is a useful method to yield basic information on the glycan structures of glycoproteins, based on the carbohydrate-binding specificities of lectins. By lectin blot analysis, researchers can directly analyze the glycan structures without releasing the glycans from glycoproteins. Here, the author describes protocols for standard analysis, and applies analysis in combination with glycosidase digestion of blot.
PMID:
25117227
DOI:
10.1007/978-1-4939-1292-6_8
[PubMed - indexed for MEDLINE]
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