Problems in silver staining of gels?

Hi, I have tried silver staining many times to stain gels, but i dont get the results. After incubating the gels in silver nitrate, i do not get any results.I have changed my silver nitrate too,...

03 April 2018 2,711 4 View

Chemically induced Glioblastoma Animal Model?

Hi, Can anyone advise me about chemically induced GBM model? How valid they are in human GBM research? Thanks

09 October 2017 7,722 1 View

Quantity of proteins from frozen cell lines and fresh culture?

Hi all, I am extracting proteins from cells frozen at -80C. Will it effect the quantity of proteins as compared to the quantity extracted from fresh cell cultures? Thankyou

07 August 2017 8,014 3 View

Bioinformatics tools for proteins?

Hi all, I need to apply some bioinformatics tools on some novel proteins and publish it. Kindly help.

06 July 2017 9,585 3 View

Can anybody check the review I have written for publication?

Hi, I have written a review entitled: ABBERANT GLYCOSYLATION OF CELL ADHESION MOLECULES (CAMs) IN PROGRESSION OF CANCER: HIGHLIGHTING GLIOMAS. Can anybody check it for me before i go for its...

05 June 2017 646 1 View

Lab groups working on proteomic analysis of Glioblastoma Multiforme??

02 March 2017 10,000 2 View

Lectin Probed Western Blotting?

I need a protocol for Lectin probed western blotting for the analysis of differentially expressed glycoproteins.

31 December 2016 135 1 View

Protein Extraction from U87 MG cell lines?

Hi, Previously, I had done protein extraction from tissue (mice brian). Now I need to extract proteins from cell lines (U87 MG). Can i follow the same protocol? If someone has a protocol or...

31 December 2016 6,575 3 View

What is the best method for Staining and Destaining the gels in SDS-PAGE?

Hi, Someone please explain me the exact solutions which can stain and destain the gels after SDS-PAGE.

31 December 2016 5,241 11 View

Cell lines as control against Glioblastoma cell lines (U87)?

Hi, I am working on Glioblastoma cell lines (U87) and looking for aberrantly expressed glycosylated proteins. which cell lines can I use as control? Can I use normal mice brain tissues as control?

11 December 2016 4,572 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View