In relation to your experience what is the best Ovarian Cell Line to work with?
Thank you!
Hi Everybody, I am planning on working with packaging and envelope plasmids (Pax2 and VSVG) and cell lines engineered to express Cas9. The biosafety committee in our institution is expecting...
05 June 2019 6,384 3 View
I would like to know if there is someone with previous experience in injecting activated human macrophages in xenograft mice? Any suggestions?
11 December 2015 8,593 1 View
I am having a problem lately. I can't obtain enough protein from cells. I am working with different cell lines and from 1.000.000 cells, I usually obtain 5-10 mg/ml of protein and that is a very...
05 June 2014 3,970 12 View
I am following step by step a published protocol but I am having some problems to isolate Sertoli cells. See attached a picture of Day 1 culture. Could anyone with experience help me to understand...
03 April 2013 9,556 5 View
I would like to interrogate a database with info about gene up-regulated and down-regulated in testicular cancer patients.
11 December 2012 1,955 2 View
I would like to make a primary culture of mouse Sertoli cells. I know Sertoli cells are very difficult to obtain in primary culture, but was wondering if there is anyone with experience in doing that?
11 December 2012 4,123 8 View
It seems very difficult to find a good sertoli cell line to work with, any suggestions?
10 November 2012 2,570 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View