Non-replicating virus: evidence to Biosafety Committee?

Hi Everybody, I am planning on working with packaging and envelope plasmids (Pax2 and VSVG) and cell lines engineered to express Cas9. The biosafety committee in our institution is expecting...

05 June 2019 6,289 3 View

Does anyone have experience in injecting activated human macrophages in xenografts mice?

I would like to know if there is someone with previous experience in injecting activated human macrophages in xenograft mice? Any suggestions?

11 December 2015 8,513 1 View

Can anyone help with protein extraction with cell line?

I am having a problem lately. I can't obtain enough protein from cells. I am working with different cell lines and from 1.000.000 cells, I usually obtain 5-10 mg/ml of protein and that is a very...

05 June 2014 3,886 12 View

Sertoli Primary Culture (Technical Problems)

I am following step by step a published protocol but I am having some problems to isolate Sertoli cells. See attached a picture of Day 1 culture. Could anyone with experience help me to understand...

03 April 2013 9,480 5 View

Which is the best database to interrogate for having information of down-regulated and up-regulated genes in testicular cancer patients/survivors?

I would like to interrogate a database with info about gene up-regulated and down-regulated in testicular cancer patients.

11 December 2012 1,889 2 View

Does anyone have a reliable protocol to isolate Sertoli cells from mice (primary culture)?

I would like to make a primary culture of mouse Sertoli cells. I know Sertoli cells are very difficult to obtain in primary culture, but was wondering if there is anyone with experience in doing that?

11 December 2012 4,055 8 View

Can anyone suggest a good sertoli cell line to work with?

It seems very difficult to find a good sertoli cell line to work with, any suggestions?

10 November 2012 2,499 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View