I would like to make a primary culture of mouse Sertoli cells. I know Sertoli cells are very difficult to obtain in primary culture, but was wondering if there is anyone with experience in doing that?
Here is a good protocol. Works well in mice.
Anway MD, Folmer J, Wright WW, Zirkin BR. Isolation of sertoli cells from adult rat testes: an approach to ex vivo studies of Sertoli cell function. Biol Reprod. 2003 Mar;68(3):996-1002.
Thank you. I was reading this paper but I was not sure if ok for mice.....Cheers
Hi
You can also find protocols here: Influence of 5α-dihydrotestosterone and 17β-estradiol on human Sertoli cells metabolism.
P F Oliveira, M G Alves, L Rato, J Silva, R Sá, A Barros, M Sousa, R A Carvalho, J E Cavaco, S Socorro. International journal of andrology. 08/2011; 34(6 Pt 2):e612-20.
Metabolic modulation induced by oestradiol and DHT in immature rat Sertoli cells cultured in vitro.
Luís Rato, Marco G Alves, Sílvia Socorro, Rui A Carvalho, José E Cavaco, Pedro F Oliveira. Bioscience reports. 06/2011; 32(1):61-9.
Good Luck
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Thanks but I do not need to work with a Sertoli cell line anymore now. Regards
I can only tell you that is a very painful procedure. I could not have good yield and cells died straight after the culture. I also spoke with other researcher and they confirmed that sertoli cell culture is very unstable. However, you can try of course. I would recommend the protocol from Oliveira et all. See above. Good luck.
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