8 Questions 24 Answers 0 Followers
Questions related from Francesco Elia Marino
Hi Everybody, I am planning on working with packaging and envelope plasmids (Pax2 and VSVG) and cell lines engineered to express Cas9. The biosafety committee in our institution is expecting...
06 June 2019 6,360 3 View
I would like to know if there is someone with previous experience in injecting activated human macrophages in xenograft mice? Any suggestions?
12 December 2015 8,567 1 View
I am having a problem lately. I can't obtain enough protein from cells. I am working with different cell lines and from 1.000.000 cells, I usually obtain 5-10 mg/ml of protein and that is a very...
06 June 2014 3,943 12 View
I am following step by step a published protocol but I am having some problems to isolate Sertoli cells. See attached a picture of Day 1 culture. Could anyone with experience help me to understand...
04 April 2013 9,533 5 View
In relation to your experience what is the best Ovarian Cell Line to work with?
03 March 2013 9,677 1 View
I would like to interrogate a database with info about gene up-regulated and down-regulated in testicular cancer patients.
12 December 2012 1,932 2 View
I would like to make a primary culture of mouse Sertoli cells. I know Sertoli cells are very difficult to obtain in primary culture, but was wondering if there is anyone with experience in doing that?
12 December 2012 4,105 8 View
It seems very difficult to find a good sertoli cell line to work with, any suggestions?
11 November 2012 2,549 1 View
