Hi Everybody,

I am planning on working with packaging and envelope plasmids (Pax2 and VSVG) and cell lines engineered to express Cas9. The biosafety committee in our institution is expecting experimental evidence of the lentivirus used in our experiment. Briefly, I was asked to test the supernatant of my cells to be sure that there are no viral particles, or better, evidence of non replicative potential. Does anyone know the best approach for providing this evidence. I was planning on using the supernatant of my cells (1 day post infection) and extract the nucleic acids. However, what is the best downstream application I could use to show the virus is non replicative and safe to use for injecting infected cells into murine models?