Non-replicating virus: evidence to Biosafety Committee?

Hi Everybody, I am planning on working with packaging and envelope plasmids (Pax2 and VSVG) and cell lines engineered to express Cas9. The biosafety committee in our institution is expecting...

05 June 2019 6,289 3 View

Can anyone help with protein extraction with cell line?

I am having a problem lately. I can't obtain enough protein from cells. I am working with different cell lines and from 1.000.000 cells, I usually obtain 5-10 mg/ml of protein and that is a very...

05 June 2014 3,886 12 View

Sertoli Primary Culture (Technical Problems)

I am following step by step a published protocol but I am having some problems to isolate Sertoli cells. See attached a picture of Day 1 culture. Could anyone with experience help me to understand...

03 April 2013 9,480 5 View

Can you suggest a good Ovarian Epithelial Cell Line to work with?

In relation to your experience what is the best Ovarian Cell Line to work with?

02 March 2013 9,621 1 View

Which is the best database to interrogate for having information of down-regulated and up-regulated genes in testicular cancer patients/survivors?

I would like to interrogate a database with info about gene up-regulated and down-regulated in testicular cancer patients.

11 December 2012 1,889 2 View

Does anyone have a reliable protocol to isolate Sertoli cells from mice (primary culture)?

I would like to make a primary culture of mouse Sertoli cells. I know Sertoli cells are very difficult to obtain in primary culture, but was wondering if there is anyone with experience in doing that?

11 December 2012 4,055 8 View

Can anyone suggest a good sertoli cell line to work with?

It seems very difficult to find a good sertoli cell line to work with, any suggestions?

10 November 2012 2,499 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View