Until now we had stored our cryopreserved tissues inside a tank with LN, but due to the high cost of this and the limiting space, we are wondering if it's possible to store those samples at -80 degrees instead.
Thank you
Dear Vanesa Cano,
The temperature for storage will depend on the tissue:
Bone and tendons need to be stored below -40°C for the full shelf life. However, they can be stored between -20° and -40°C for up to 3 months.
Amnion must be stored below -40°C as the high concentration glycerol it is stored in liquefies above this temperature. Once thawed, they can be placed in a +4°C refrigerator for up to 24 hours. Do not re-freeze.
Cryopreserved tissue e.g. skin, heart valves
This tissue is stored in vapor phase nitrogen at below -135°C at Tissue and Eye Services and is usually supplied in dry ice (-79°C). It should not be returned to nitrogen storage. This product can be stored in a dedicated alarmed freezer running at -80°C until the expiry date (permitted 6 months storage). Once thawed, it can be placed in a +4°C refrigerator for up to 24 hours. Do not re-freeze.
Sure..can stored the tissues at -80oC but the survival the tissues maybe 3 until 6 month only
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