How to generate .dlg files on PyRx?

Hi. I'm using the PyRx version 0.8 and getting the error message: WARNING: Filename mismatch: "2DFN_ADP_CL.maps.fld" :: "..\2DFN_ADP_CL.maps.fld" C:\Program Files (x86)\The Scripps Research...

17 January 2021 843 3 View

Which is the viscosity recommended for using Methyl cellulose to perform oral gavage (mice )?

Hello, we would like to perform oral gavage in mice. The vehicle recommended for our drug consist on 0.5% methylcellulose and 5% Dimethylacetamide (0.1 ml/ 10g mouse). Could somebody help us to...

20 August 2020 8,720 2 View

Can normal colon cell lines form spheroids?

I am growing spheroids from HT29 colon cancer cells, and I need some sort of control cells to be compared with the HT29. Now, I should grow the control cell into spheroids and then use these new...

30 October 2018 3,719 12 View

Why do I have Inhibition in RNA extracted from Root bacteria ?

25 April 2017 883 6 View

New subunit E2 vaccine with celular respond after 7 days of immunization, one dose could be important?

I will appreciate very much your opinion

05 May 2016 3,366 1 View

Antibody specific for POTEF or POTEE for IHC?

I am looking for an antibody specific for POTEF or POTEE proteins (120KDa) for IHC. I have only found antibodies for FACS, WB and ELISA, and some of them are not 100% specific (bind to small...

21 April 2016 3,513 1 View

Does anyone have any idea of the half-life of DKK1 and sFRP3?

In my experiments, time has became an important variable associate to these proteins levels

01 January 2016 5,121 4 View

Do we need to keep frozen cell immersed or just above the liquid nitrogen level?

04 December 2014 6,239 4 View

What is information quality?

This is a very interesting think to search about. I would like to receive articles recomendations about this theme. Thanks!

28 October 2011 1,318 2 View

co-culturing adherent and non-adherent cells

19 April 2011 7,805 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View

How to calculate the sample size in an interobserver variability study?

Hi I am conducting an interobserver variability study where we have 12 raters who are going to rate samples of lesions. They will rate up to 10 variables per sample. Although the size of the...

12 February 2021 7,276 3 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

I am looking for an antibody to Arc protein for use in immunohistochemistry with rhesus monkey brain tissue?

Looking for a well-validated antibody to detect Arc protein expression in paraformaldehyde fixed brain tissue using immunohistochemistry. Ideally this antibody would have been published on using...

07 February 2021 8,457 1 View