Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View

How to preserve wet fungus spore ?

I need to preserve broth culture of wet fungus spore for my further research. Can any one suggest me any solution.

18 January 2021 4,756 2 View

Does long term preservation (10years) of Collembola in 99% Ethanol affect recovery rate of extraction by flotation?

Hello! I am currently developing an automated counting approach using Fiji to count Collembola from pitfall trapped samples that have been stored in Ethanol (99%) since 2010. I use flotation (tap...

13 January 2021 659 2 View

Cryopreservation of cancer cell lines @-80?

07 January 2021 5,410 8 View

How to select/separate mononuclear cells in a plurinuclear population cells?

Hi, I'm working with a fungal specie wich have plurinuclear cells (conidia or hyphae or protoplasts have between 1 (< 5%) to 8 nuclei however majority have 4-6 nuclei ). I have made deletion...

06 January 2021 9,154 4 View

Matlab: train random forest with time series? How to transform time series data to a supervised learning dataset?

Hey, I've build random forest using Matlab Machine-Learning Toolbox Function (treeBagger). I've computed several kinematic features like velocity or acceleration as predictors (24 predictors) for...

22 December 2020 2,638 3 View

How to do Fusarium graminearum protoplast transformation using desired plasmid?

I would like to specify my question regarding the transformation of plasmid DNA into F graminearum protoplast. I am using the following reaction mixture (30%PEG 50ul, 200ul protoplast, and 20ug of...

14 December 2020 4,251 1 View