I am purchasing human PBMC stocks at 2.5 x 10^7 cells/ml. That is too high of a conc. to work with and I was wondering once thawed, can I refreeze smaller aliquots down?
Neha Rana I am going to try thawing out my stock and immediately re-freezing it back in DMSO/FBS without growing in media and assess. I'm planning to do it next week - I can keep you updated :)