The main expenses incurred in plant tissue culture is the sucrose and agar. Therefore there is always a search for a cost effective solution for the same as plant tissue culture needs numerous trail and error experiments.
Yes it can be. One of my colleague had tried it in my lab and it has worked. Well i do not know the concentration and the conc also depend on the plant you are working. You can get an idea through the attached paper.
From this research below, you should be able to use table sugar, but tissue-culture grade sucrose can be superior when certain amount of them are used.
Please take a look at the attached paper for an example of: Table sugar vs. Lab-grade sucrose in plant tissue culture.
Title: Comparative study of effects of table sugar, laboratory-grade sucrose and mannitol on growth of banana plantlets under in vitro conditions.
Partial of the study results:
"The laboratory-grade sucrose showed the highest effect to support the growth of banana plantlets under in vitro condition at a level of 30g x L-1. However, reducing sugar levels to 15g x L-1 of culture medium results in less weight gain and growth. Table sugar was able to support growth of the banana plantlets to the same level as laboratory sucrose at 15g x L-1 . Therefore, to reduce the production cost of in vitro plantlets the table sugar can be used. "
My laboratory routinely use table sugar for micropropagation purposes, instead of tissue culture grade sucrose. We also use commercial food agar (the version without food color or sweetener) for solidifying agents. However, for publication purpose - I would not recommend the use of table sugar since it might be questionable to reviewers.
For common micropropagation purposes, I use 30 g of table sugar and 6-7 g of commercial food agar. It work fine for a number of horticultural crops in Indonesia. Good luck.
yes, you can do it!!! depend of tissue you work your concentration, 1-2% en vegetal tissue and 2-3 % in callus, but with more sugar (carbohidrate source) it is possible there are more bacterial contamination
I used table sugar for several years in commercial production of strawberries, apple trees and Alnus species. I had first made an analysis of table sugar before and after analysis and all there was in it was saccharose. Since I found no difference between table sugar and lab grade saccharose (at least in Canada, the brand name is Lantic), I was using the same concentration as with the lab grade. I never had any problem using it in every steps of the micropropagation process.
Sugar can be added as substitute. it is used generally 3 gm/ltr. Best to use I.P. or B.P. grade sugar. if not then atleast sulphurless.
I have been using cane sugar since long ago, especially for stock cultures, at concentration of 3%, without any apparent problem.
Yes, our recent tissue culture experiments have been working fine with table sugar, though we use tissue culture grade sucrose for critical experiments. Our standard concentration has been 30g per liter.
Yes! you can very well use market grade table sugar for tissue culture work. Your need to depend on dgades like Dhampur sugar or Trust sugar (In India). The concentration is same 2-3 % depending upon your requirement
I used table sugar for mass propagation in strawberry at concentration of 3% without any problem.
Yes off course. You can try. I have tested it in some plants for shooting and rooting and I found its results satisfactory. It could be a better a cheap option for making plant tissue culture cost effective.
Interesting question. The carbs in a spoon of sugar is about 4 grams. When cane sugar is raw there is no fat, protein, and cholesterol. There are aspects of nutrition more on a micro basis.
References
Hula Foods (2020). Health benefits of raw sugar cane. Retrieved from: https://www.hulagirlfoods.com/organic-sugar-cane-benefits/health-benefits-of-raw-sugar-cane/
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