Anil Kumar

3 Questions 22 Answers 0 Followers

Questions related from Anil Kumar

Any suggestions regarding the southern blotting using DIG labelled probe?

We are trying to find the copy number of transgenes in a Eucalyptus plant using DIG labelled probe (700 bp) and color detection. We are using Roche labeling and Detection Kit. However we are...

31 May 2013 7,981 1 View

Could someone please let us know which of the following techniques (southern blot or Real-Time PCR) is better for determining copy number of transgene?

Transgenic plants.

09 November 2012 309 2 View

Can some please suggest a method to calculate the copy number of a transgene using real-time PCR

We have undertaken the genetic transformation of Eucalyptus. We want to find the copy number of the transgene in the different transgenic lines. Could anyone please explain this using Real-Time...

25 October 2012 9,901 5 View