can someone kindly help me to know how to prepare double charcoal stripped serum from normal serum and charcoal?
Hello to all Does anyone have reliable information about the chemical properties of methanol and the combination of methanol with water? With valid reference We need this information to select the...
25 December 2023 8,901 4 View
What is the difference between vector pET (26,21, a,,b,+.........? What are the changes made in the PET vector (for different pET)?
06 December 2023 5,576 1 View
I will be grateful for any advice in this regard.
16 July 2022 5,416 0 View
Hi every body, I want to fabricated a supercapacitor electrode with Ti3C2Tx (MXene) on carbon fiber. my question is : How can I encapsulated carbon fibers to avoid capillary effect of the...
12 July 2022 3,843 0 View
Hi everyone ,I am working on three autophagy genes I want to calculate the efficacy so I dilute my samples in this way:1/10,1/100,1/1000 and so on.But the dilutions more than 1/1000 have primer...
19 April 2022 5,353 1 View
Hi researchers! I have gained something strange in the result of mtt assay of the PBMC. I was testing an herbal extract that is declared to be safe for normal blood cells by researchers but I saw...
13 April 2022 5,323 2 View
Hi researchers I am doing real time for some autophagy genes . At first some of them didn‘t have any bonds or had primer dimer!! So I have reduced primer concentration ,increased the template...
04 April 2022 6,436 3 View
Hi researchers! I want to run a mtt assay on PBMC to show that a cytotoxic drug in a leukemia, does not have any effect on normal cells I mean PBMC! Is there any special protocol to isolate ,seed...
02 April 2022 3,637 3 View
Hi researchers, I was searching for the synergism of two different drugs by running mtt assay,I analyzed my data and I found them synergism, so I used each drug IC50 and both Ic50 to check them by...
17 December 2021 6,329 3 View
Hi everybody, I found already a few papers explaining the Gro-seq experiment. Since this experiment is sensitive and complicated, Can someone introduce a kit to make this experiment comfortable.
11 December 2021 7,206 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
How Social Media Affects Your Mental Health
04 August 2024 6,961 3 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Hello, Dear friends, I am surveying as part of my thesis research and would greatly appreciate your participation. The study investigates how social media influences dental health behaviors...
01 August 2024 9,349 2 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View
I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...
01 August 2024 8,754 2 View