I will be grateful for any advice in this regard.
Hi everybody, I am doing CHIP experiment. The problem is that in my gel agarose I see alot of DNA with big size (longer that 1000 bp) which means there is alot of unfragmented DNA in my gel. I...
06 September 2020 1,507 5 View
Hello everyone, I see a lot of papers with the spheroid generation topic. I know spheroids are used widely for simulation of tumor condition in body. Can someone say which abilities does the...
31 August 2020 3,323 3 View
Hi everybody, I have cultivated the C4-2 cell line in double charcoal stripped serum containing medium. A lot of the cells do not attach to the bottom of the flask. If someone has experience...
24 August 2020 9,634 2 View
I am culturing C4-2 cells in 15 cm dishes in different serumes of 5% FCS and 5% DCSS. since the cells are slow in attaching to the dish in DCSS compare to the FCS if someone has experience, can...
20 August 2020 1,535 1 View
Hi everybody, I wanna use an antibody to determine the presence of a protein in my chip-seq experiment. Can someone help me how can do concentration optimization for antibody which I use ? Thanks...
19 July 2020 9,619 3 View
Assuming that non-durable goods last less than three years on average, which direction do you think the world businesses and consumers are moving considering the huge environmental impact of...
27 May 2020 4,062 4 View
The industrial world is confronting the environmental side of sustainability. But, how do you evaluate the incentives - either from society or government- to convince them about sustainability...
09 May 2020 557 47 View
Hi everybody, Does anybody have experiences like getting different lifetime result by changing magnification from a sample? Can this factor lonely has influence on the lifetime of a tissue? Bests
20 March 2020 8,745 3 View
Hi everyone, I currently did some experiments with a pig serum (notnheat inactivated), which the control negative of that showed bacterial contamination. As I have to filter it befor using and I...
01 December 2019 8,649 3 View
I extract the primary RPE cells from pig. As I see in several time extraction high contamination of bacterial in my cell culture flask in the 3rd day after extraction. Sometimes they survive...
18 November 2019 8,623 5 View
Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...
24 February 2021 9,902 5 View
during MD simulation, the atoms are not moving as per required. The problem (as per my observation) lies in energy minimization technique. upon application of tensile strain in x or y direction,...
22 February 2021 380 5 View
I am working on one optimization problem. the model with two states, the transition between the two states based on one threshold value (L = 5, which L< 5 -> Normal state, L== 5 ->...
11 February 2021 4,045 3 View
i am trying to formulating one formulation but the problem i am facing is after formulating and adjusting it's pH, when i try to check pH after 7 days it is getting down towards the acidic side....
02 February 2021 9,343 3 View
Hello Everyone! I have prepared liposomal formulation using Rotary Evaporator method followed by dialyzed the liposomal Suspension in Phosphate buffer to remove the un-encapsulated drug. After...
25 January 2021 7,752 4 View
Hello everyone. I'm a pharmaceutical scientist whose experience is exclusively in solid dosage form. I'm starting a project that involves a semi-solid (cream) formulation, but I can't find a lot...
24 January 2021 4,055 3 View
Need to have a validated, stable method to separate tricholine citrate and cyproheptadine hydrochloride from a syrup formulation.
20 January 2021 4,216 2 View
I want to measure thickness of screen printed graphene ink on paper substrate. Can anyone suggest any accurate and feasible method to measure it. I tried profilometry (stylus & optical) but...
11 January 2021 6,179 6 View
The realistic approach to identify the exact choices and or preferences is quite difficult seems due to multiple unrealistic profiteering triggers applied to confuse and deviate the end-users from...
25 December 2020 8,304 1 View
I need to formulate the lagrangian dynamic equations for the hexapod robot's body. The body will constitute 6 DoF, which is 3 linear and 3 angular. I have the coordinates of each corner of the...
24 December 2020 1,222 1 View
