Does anyone know how to remove primer dimer in standard curve of Real time?

20 April 2022 1 5K Report

Hi everyone ,I am working on three autophagy genes I want to calculate the efficacy so I dilute my samples in this way:1/10,1/100,1/1000 and so on.But the dilutions more than 1/1000 have primer dimers in melt curve,Is there anyway to calculate the efficacy of primers?!

Elmira Zarei

Please anyone can help me with primer dimer in standard cuvre?!

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Science method

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