Elmira Keramatfar

12 Questions 5 Answers 0 Followers

Questions related from Elmira Keramatfar

Can someone guide me how can I solve this problem In first step of my CHIP experiment?

Hi everyone, In order to do CHIP experiment, I used some lysis buffer and micrococcus followed by sonication to digest chromatin of the cells. I did all the process of cross-lincking, quenching,...

12 September 2020 1,433 3 View

Can someone help me to how to optimize the chromatin digestion for my CHIP experiment?

Hi everybody, I am doing CHIP experiment. The problem is that in my gel agarose I see alot of DNA with big size (longer that 1000 bp) which means there is alot of unfragmented DNA in my gel. I...

07 September 2020 1,628 5 View

Can someone explane the difference between spheroids generated from primary cells and spheroid produced from cell line?

Hello everyone, I see a lot of papers with the spheroid generation topic. I know spheroids are used widely for simulation of tumor condition in body. Can someone say which abilities does the...

01 September 2020 3,442 3 View

Does anybody know the difference of the effect of the dextran coated charcoal stripped serum (DCSS) containing medium and DCSS containg medium?

Hi everybody, I have cultivated the C4-2 cell line in double charcoal stripped serum containing medium. A lot of the cells do not attach to the bottom of the flask. If someone has experience...

25 August 2020 9,789 2 View

Does someone have experience with cultivation of C4-2 cells in 15 cm dishes?

I am culturing C4-2 cells in 15 cm dishes in different serumes of 5% FCS and 5% DCSS. since the cells are slow in attaching to the dish in DCSS compare to the FCS if someone has experience, can...

21 August 2020 1,687 1 View

Can someone explain how we can Ioptimize antibody concentration which I wanna use in CHIP-qpcr?

Hi everybody, I wanna use an antibody to determine the presence of a protein in my chip-seq experiment. Can someone help me how can do concentration optimization for antibody which I use ? Thanks...

20 July 2020 9,752 3 View

Has someone experience about getting different lifetime result by changing magnification of a sample?

Hi everybody, Does anybody have experiences like getting different lifetime result by changing magnification from a sample? Can this factor lonely has influence on the lifetime of a tissue? Bests

21 March 2020 8,863 3 View

Can someone suggest a sterilized, clean and trustable pig serum company for ordering?

Hi everyone, I currently did some experiments with a pig serum (notnheat inactivated), which the control negative of that showed bacterial contamination. As I have to filter it befor using and I...

02 December 2019 8,770 3 View

How much is the possible maximum dose for cells without showing toxic effect?

I extract the primary RPE cells from pig. As I see in several time extraction high contamination of bacterial in my cell culture flask in the 3rd day after extraction. Sometimes they survive...

19 November 2019 8,740 5 View

Does anybody cultured primary RPE cells in higher antibiotich concentration of 2%?

I did primary RPE cells extraction from the fresh pig eyes, but after 3 days of seeding cells the medium (miller medium including 10% pig serum and 2% penicillul/streptomycin) is looking cloudy(...

11 November 2019 7,319 3 View

Can someone guide me how can i find the location of sample when I switch my microscop in bright field to the spectral imaging setting?

Hi everyone, Usually finding the sample position in bright field is easy but when we change the microscop to the spectroscopy setting, my sample got lost. Does anybody has a solution for this...

16 September 2019 6,717 3 View

How can I improve my MTF inreverse Eye model optimization in Zemax?

Dear colleagues, I have a revers eye model in zemax which I should optimize it to reach the paper mentioned curve of MTF( by changing conic constant of back lens and thicckness of vitreous) but...

08 February 2019 9,918 2 View