Is there any literature showing the change in resident fibroblasts composition of the human heart with age?

I am interested to study cardiac fibroblasts in detail regarding the phenotype, differentiation and proliferation. I was curious if there is literature with cardiac composition change in...

31 December 2019 1,227 1 View

Are gap junction proteins (Cx43/Cx40/Cx45) expressed in prenatal rat hearts?

I have tried western blot and immunocytochemistry for connexins (40,43,45) which are said to be predominant connexin isoforms in mammalian cardiomyocytes. However, there aren't any traces of them...

02 March 2019 282 2 View

Can you suggest a working protocol to block Cx43-gap junctions in cardiomyocytes in vitro?

With reference to a publication on Inhibiting Cx43 Gap junctions in cardiomyocytes using chemical compounds such as Ioxynil octanoate, I would like to inhibit gap junctions and image live-cell...

07 August 2018 4,301 1 View

Is it possible to store rat prenatal heart tissue for future isolation of cardiomyocytes?

I usually isolate cardiomyocytes immediately from the hearts extracted from prenatal rats. But I would like to know if it is possible to store them in a medium for future isolation. Please suggest...

06 July 2018 5,336 1 View

Is connexin43 really present in cardiac fibroblasts?

Its very clear that the myocytes have Cx43 but what about the fibroblasts? And if so what is its role in the heart?

09 October 2016 3,758 1 View

What is the effect of uv laser on Fluo4AM calcium dye?

09 October 2016 133 1 View

Hi fellow researchers. I isolated neonatal rat cardiomyocytes for primary culture studies. But only few clusters beat in culture. Whats the reason?

05 June 2016 6,941 4 View

Can someone provide me the standard protocol for rat cardiac connexin40/43/45 immunostaining?

I would like to stain cx40,43 and 45 in rat cardiac cells. It would be good if there is a standard protocol for it. Thanks in advance

01 January 1970 1,482 1 View

Is there a standard working protocol for immunostaining of connexin43/40/45 in rat cardiac cells?

Lately I have been trying to perform immunofluorescence staining for connexins in primary rat cardiac cells but I donot visualize connexin gap junctions at all in my samples. I compared it with...

01 January 1970 8,673 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

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28 February 2021 9,936 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View