Are gap junction proteins (Cx43/Cx40/Cx45) expressed in prenatal rat hearts?

I have tried western blot and immunocytochemistry for connexins (40,43,45) which are said to be predominant connexin isoforms in mammalian cardiomyocytes. However, there aren't any traces of them...

02 March 2019 282 2 View

Can someone help me out with published literature on evidence of connexins in embryonic rats ?

I am working in with embryonic rat cardiomyocytes and I have in immunolabelling connexin proteins in the cultured cells. Right now I would like to check for the presence of gap junction proteins...

10 November 2018 6,839 0 View

Can you suggest a working protocol to block Cx43-gap junctions in cardiomyocytes in vitro?

With reference to a publication on Inhibiting Cx43 Gap junctions in cardiomyocytes using chemical compounds such as Ioxynil octanoate, I would like to inhibit gap junctions and image live-cell...

07 August 2018 4,301 1 View

Is it possible to store rat prenatal heart tissue for future isolation of cardiomyocytes?

I usually isolate cardiomyocytes immediately from the hearts extracted from prenatal rats. But I would like to know if it is possible to store them in a medium for future isolation. Please suggest...

06 July 2018 5,336 1 View

Is connexin43 really present in cardiac fibroblasts?

Its very clear that the myocytes have Cx43 but what about the fibroblasts? And if so what is its role in the heart?

09 October 2016 3,758 1 View

What is the effect of uv laser on Fluo4AM calcium dye?

09 October 2016 133 1 View

Hi fellow researchers. I isolated neonatal rat cardiomyocytes for primary culture studies. But only few clusters beat in culture. Whats the reason?

05 June 2016 6,941 4 View

Can someone provide me the standard protocol for rat cardiac connexin40/43/45 immunostaining?

I would like to stain cx40,43 and 45 in rat cardiac cells. It would be good if there is a standard protocol for it. Thanks in advance

01 January 1970 1,482 1 View

Is there a standard working protocol for immunostaining of connexin43/40/45 in rat cardiac cells?

Lately I have been trying to perform immunofluorescence staining for connexins in primary rat cardiac cells but I donot visualize connexin gap junctions at all in my samples. I compared it with...

01 January 1970 8,673 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View