Is there any literature showing the change in resident fibroblasts composition of the human heart with age?

I am interested to study cardiac fibroblasts in detail regarding the phenotype, differentiation and proliferation. I was curious if there is literature with cardiac composition change in...

31 December 2019 1,227 1 View

Are gap junction proteins (Cx43/Cx40/Cx45) expressed in prenatal rat hearts?

I have tried western blot and immunocytochemistry for connexins (40,43,45) which are said to be predominant connexin isoforms in mammalian cardiomyocytes. However, there aren't any traces of them...

02 March 2019 282 2 View

Can someone help me out with published literature on evidence of connexins in embryonic rats ?

I am working in with embryonic rat cardiomyocytes and I have in immunolabelling connexin proteins in the cultured cells. Right now I would like to check for the presence of gap junction proteins...

10 November 2018 6,839 0 View

Can you suggest a working protocol to block Cx43-gap junctions in cardiomyocytes in vitro?

With reference to a publication on Inhibiting Cx43 Gap junctions in cardiomyocytes using chemical compounds such as Ioxynil octanoate, I would like to inhibit gap junctions and image live-cell...

07 August 2018 4,301 1 View

Is connexin43 really present in cardiac fibroblasts?

Its very clear that the myocytes have Cx43 but what about the fibroblasts? And if so what is its role in the heart?

09 October 2016 3,758 1 View

What is the effect of uv laser on Fluo4AM calcium dye?

09 October 2016 133 1 View

Hi fellow researchers. I isolated neonatal rat cardiomyocytes for primary culture studies. But only few clusters beat in culture. Whats the reason?

05 June 2016 6,941 4 View

Can someone provide me the standard protocol for rat cardiac connexin40/43/45 immunostaining?

I would like to stain cx40,43 and 45 in rat cardiac cells. It would be good if there is a standard protocol for it. Thanks in advance

01 January 1970 1,482 1 View

Is there a standard working protocol for immunostaining of connexin43/40/45 in rat cardiac cells?

Lately I have been trying to perform immunofluorescence staining for connexins in primary rat cardiac cells but I donot visualize connexin gap junctions at all in my samples. I compared it with...

01 January 1970 8,673 2 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View