With reference to a publication on Inhibiting Cx43 Gap junctions in cardiomyocytes using chemical compounds such as Ioxynil octanoate, I would like to inhibit gap junctions and image live-cell calcium imaging to observe the effect. Can someone provide me suggestions?
Joseph P Stains
There are some chemicals inhibitors (e.g., carbenoxolone, heptanol, or 18alpha glycyrrhetinic acid) of questionable specificity that can be used for short term inhibition of gap junctions. There are peptide based inhibitors as well which improve specificity but require large amounts to be effective (Article Bridging the gap to therapeutic strategies based on connexin...
). Your best bet for specificity is genetic...either Gja1 siRNA knockdown or the use of cells from floxed Gja1 mice and Cre-Expression will be most convincing, assuming you get sufficient knockdown efficiency.
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