Krishna Chander Sridhar

10 Questions 4 Answers 0 Followers

Questions related from Krishna Chander Sridhar

Is there any literature showing the change in resident fibroblasts composition of the human heart with age?

I am interested to study cardiac fibroblasts in detail regarding the phenotype, differentiation and proliferation. I was curious if there is literature with cardiac composition change in...

01 January 2020 1,266 1 View

Are gap junction proteins (Cx43/Cx40/Cx45) expressed in prenatal rat hearts?

I have tried western blot and immunocytochemistry for connexins (40,43,45) which are said to be predominant connexin isoforms in mammalian cardiomyocytes. However, there aren't any traces of them...

03 March 2019 328 2 View

Can someone help me out with published literature on evidence of connexins in embryonic rats ?

I am working in with embryonic rat cardiomyocytes and I have in immunolabelling connexin proteins in the cultured cells. Right now I would like to check for the presence of gap junction proteins...

11 November 2018 6,909 0 View

Can you suggest a working protocol to block Cx43-gap junctions in cardiomyocytes in vitro?

With reference to a publication on Inhibiting Cx43 Gap junctions in cardiomyocytes using chemical compounds such as Ioxynil octanoate, I would like to inhibit gap junctions and image live-cell...

08 August 2018 4,345 1 View

Is it possible to store rat prenatal heart tissue for future isolation of cardiomyocytes?

I usually isolate cardiomyocytes immediately from the hearts extracted from prenatal rats. But I would like to know if it is possible to store them in a medium for future isolation. Please suggest...

07 July 2018 5,382 1 View

Is connexin43 really present in cardiac fibroblasts?

Its very clear that the myocytes have Cx43 but what about the fibroblasts? And if so what is its role in the heart?

10 October 2016 3,807 1 View

What is the effect of uv laser on Fluo4AM calcium dye?

I have been doing experiments on cardiac cells. For imaging i use Fluo4AM dye. But i want to know if the UV laser radiation on to the dye affects the calcium wave in the cells. Thanks in advance

10 October 2016 180 1 View

Hi fellow researchers. I isolated neonatal rat cardiomyocytes for primary culture studies. But only few clusters beat in culture. Whats the reason?

I suspect that it could be due to the loss of pacemaker cells during the isolation or is it due to the culture conditions.

06 June 2016 6,997 4 View

Is there a standard working protocol for immunostaining of connexin43/40/45 in rat cardiac cells?

Lately I have been trying to perform immunofluorescence staining for connexins in primary rat cardiac cells but I donot visualize connexin gap junctions at all in my samples. I compared it with...

01 January 1970 8,712 2 View

Can someone provide me the standard protocol for rat cardiac connexin40/43/45 immunostaining?

I would like to stain cx40,43 and 45 in rat cardiac cells. It would be good if there is a standard protocol for it. Thanks in advance

01 January 1970 1,526 1 View