Is there any literature showing the change in resident fibroblasts composition of the human heart with age?

I am interested to study cardiac fibroblasts in detail regarding the phenotype, differentiation and proliferation. I was curious if there is literature with cardiac composition change in...

31 December 2019 1,227 1 View

Are gap junction proteins (Cx43/Cx40/Cx45) expressed in prenatal rat hearts?

I have tried western blot and immunocytochemistry for connexins (40,43,45) which are said to be predominant connexin isoforms in mammalian cardiomyocytes. However, there aren't any traces of them...

02 March 2019 282 2 View

Can someone help me out with published literature on evidence of connexins in embryonic rats ?

I am working in with embryonic rat cardiomyocytes and I have in immunolabelling connexin proteins in the cultured cells. Right now I would like to check for the presence of gap junction proteins...

10 November 2018 6,839 0 View

Can you suggest a working protocol to block Cx43-gap junctions in cardiomyocytes in vitro?

With reference to a publication on Inhibiting Cx43 Gap junctions in cardiomyocytes using chemical compounds such as Ioxynil octanoate, I would like to inhibit gap junctions and image live-cell...

07 August 2018 4,301 1 View

Is it possible to store rat prenatal heart tissue for future isolation of cardiomyocytes?

I usually isolate cardiomyocytes immediately from the hearts extracted from prenatal rats. But I would like to know if it is possible to store them in a medium for future isolation. Please suggest...

06 July 2018 5,336 1 View

Is connexin43 really present in cardiac fibroblasts?

Its very clear that the myocytes have Cx43 but what about the fibroblasts? And if so what is its role in the heart?

09 October 2016 3,758 1 View

Hi fellow researchers. I isolated neonatal rat cardiomyocytes for primary culture studies. But only few clusters beat in culture. Whats the reason?

05 June 2016 6,941 4 View

Can someone provide me the standard protocol for rat cardiac connexin40/43/45 immunostaining?

I would like to stain cx40,43 and 45 in rat cardiac cells. It would be good if there is a standard protocol for it. Thanks in advance

01 January 1970 1,482 1 View

Is there a standard working protocol for immunostaining of connexin43/40/45 in rat cardiac cells?

Lately I have been trying to perform immunofluorescence staining for connexins in primary rat cardiac cells but I donot visualize connexin gap junctions at all in my samples. I compared it with...

01 January 1970 8,673 2 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Where can find Medical Image Data Sets for Machine learning research project?

I want to work on medical image(disease) detection and classification. Where can I found Image data sets?

24 February 2021 3,130 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

What is your preference for journals for publishing in the field of Hyperspectral Imaging or Remote sensing?

I believe that we need to share our experience in publishing in the field of Hyperspectral Imaging or remote sensing? What is the best journal that you publish with? What is the worst experience...

23 February 2021 3,411 4 View

Could the "incoming radiation (net radiation)" values be negative?

Could the "incoming solar radiation" values be negative? What is the difference between "global solar radiation" and "incoming solar radiation"? Which one might be measured negative values?

21 February 2021 7,726 9 View