I am looking for treatments and/or perturbations, which may include small molecule compounds, antibodies, siRNA, Crispr targets, and other KD/KO inducers. I need some suggestions as to which databases I could refer to for the same.
Thanks in advance
Hi all, I'm working on MDCK II cell line where I grow the cells on PDMS substrate into a dense tissue (about 5mm diameter in size). I have been trying out different plasma membrane dyes for my...
14 January 2024 9,842 0 View
We have the diameter of the core nanoparticles.
19 November 2023 3,113 3 View
The brain activity during individual images will be analyzed post-hoc for correctly recognized/ forgotten pictures during retention a few days later. Currently, we have 150 images; 3 seconds for...
17 September 2023 7,836 0 View
I have been trying to stably knockdown a gene in mammalian cells using viral supernatant from HEK293FT cells. Although I have found transfection efficiency to be good enough multiple times, after...
27 August 2021 7,084 1 View
Hello, my work involves growing cells in the matrigel and for recovering cells from matrigel i usually use cell recovery solution from corning. As now i am curious to know if theres an alternate...
20 November 2020 1,484 3 View
I am observing a large necrotic population in glioblastoma cells that I am culturing in 10% DMEM medium. In spite of changing all parameters of culturing and making sure of the sterilitu, the...
05 November 2020 9,445 3 View
I am trying to conjugate a 12 amino acid peptide (MW 1565Da) to PLGA-PEG-maleimide copolymer (MW: 35097Da). The conjugation reaction takes place in ACN: DMF as a solvent. Can someone please...
19 October 2020 825 5 View
I have yeast cell lysate transformed with a virus. I want to analyse the viral proteins by using the BCA assay. How would I do that? (Lysing the yeast cells would release the yeast proteins also...
01 October 2020 5,058 3 View
I am trying to model a Benzene recovery process by extractive distillation in Aspen plus. The calculation type chosen for the radfrac column is 'equilibrium' with the tray efficiency of 75%. i...
04 February 2020 9,419 3 View
I am gonna grow hacat and L929 cells and can i grow these cell line with dmem high glucose+ 4mM L glutamine? Which is more preferable 2mM or 4mM glutamine? Thanks in advance.
09 March 2019 4,271 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.
25 July 2024 8,313 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View