Hi all,
I'm working on MDCK II cell line where I grow the cells on PDMS substrate into a dense tissue (about 5mm diameter in size). I have been trying out different plasma membrane dyes for my live imaging experiments but none of them seem to work that well. I either see dull signal with a lot of background or I see nothing. I tried cellmask plasma membrane stain, cellbrite, and Biotracker cell membrane dye. It's also that the laser has to go through the PDMS gel thickness to image the cells. Recently, I tried cellbrite on my cells grown on glass substrate which gave me really good signal and I am really happy with the result but the cells grown on the gel is where I can't get a clear signal. Have you ever had this problem? Any suggestions would be really help me.
Thanks for all your suggestions !!!
Best,
Madhura
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