Raman Spectroscopy has emerged as a major technology for easy qualitative detection of organic molecules. In biotechnology, DNA sequences are important as they represent vital information. In this context, if we know structure of particular sequence of nucleotides, can we use ramanspectroscopy to detect presence of the sequence in the unknown DNA extract.
An allele is positioned on a locus, the specific location of gene or DNA sequence on a chromosome, so the............. Two groups of tests, molecular and cytogenetic, are used in genetic syndrome. Raman Spectroscopy, a chemically specific label free diagnostic is rapidly gaining in acceptance as a powerful technique. For further details consult Curr Opin Biotechnol. 2009 Feb: 20( 1 ): 63 - 73.
Well, yes, good idea. The specificity of Raman spectroscopy is limited, anyway it can show specific structural motives. Depending on the complexity I'd suggest to use more traditional tools based on amplification such as PCR, qPCR, or isothermal amplification, microarrays, sequencing, etc. In other words to stay in the realm of the "classical" molecular biology.
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