Effect of flash freezing of pelleted cells?

01 February 2017 1,646 3 View

Are there any post translational modifications that include addition of a thiol?

I know there are PTMs that make use of the thiol group on cysteine, but does anyone know of any PTMs that add a thiol group to a protein (in any organism, not just humans)?

02 March 2013 1,323 1 View

What controls are needed in immunoprecipitation?

I have a custom antibody (it works for Western blot and immunohistochemistry) that I would like to try for immunoprecipitation. I know that the antibody successfully binds my beads (I'm...

02 March 2013 4,804 7 View

Chemical lysis of coelomocytes?

Does anyone know of a chemical lysis method for total protein isolation from coelomocytes? I'm currently using physical disruption in the presence of detergent. This gets me a fair amount of my...

31 December 2012 4,234 5 View

How can you evaluate EDU (labeling of newly synthesized DNA) in cells that are non adherent without flow cytometry?

We are studying cell proliferation in a cell type (from a marine invertebrate) that is non-adherent. Does anyone have suggestions for method(s) to evaluate EDU labeling in non adherent cells? We...

08 September 2012 3,714 17 View

Good tool for qRTPCR primer design?

I'm designing some primers for qRTPCR. Does anyone have any suggestions for a good qRTPCR primer design tool? (I expect to need to check for hairpins and dimers and what not, but would prefer...

08 September 2012 1,395 5 View

Suggestions for immunoprecipitation kits/protocols?

I'm somewhat new to IP and am looking for a good kit (or bead type) to isolate my proteins (large family of related proteins; we have an antibody specific for a conserved region). My antibody is a...

07 August 2012 5,033 5 View

How to evaluate EDU in cells that are non-adherent without flow cytometry?

We are studying cell proliferation in a cell type (from a marine invertebrate) that is non-adherent. Does anyone have suggestions for method(s) to evaluate EDU labeling in non adherent cells?...

07 August 2012 598 1 View

Has anyone tried using Bio-Rad Criterion XT gels with a home-made running buffer?

I'm looking to buy gels for the second dimension of IEF, and am attracted to the Bio-Rad Criterion XT gels - largely for their relatively long shelf-life (either the Bis-Tris with MOPS running...

07 August 2012 3,491 12 View

How can I quantify total protein concentration when a colored contaminant is present in my sample?

I am isolating a protein by metal affinity chromatography, concentrating and desalting it, and then drying it by vacuum centrifugation. I would like to be able to quantify my protein at various...

05 June 2012 9,987 61 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

No title

02 March 2021 3,060 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View