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Questions related from Lauren S Sherman
EDIT: I KNOW IT ultimately RESULTS IN CELL LYSIS. My question is how? Is it just crystal formation? It seems to me that snap freezing the cells results in a sensitization of sorts of the cell...
02 February 2017 1,695 3 View
I am trying to grow mesenchymal stem cells on a glass coverslip (to be used for confocal microscopy). Does anyone have any experience with growing (not simply adhering) MSCs to glass coverslips?...
11 November 2014 7,220 4 View
I know there are PTMs that make use of the thiol group on cysteine, but does anyone know of any PTMs that add a thiol group to a protein (in any organism, not just humans)?
03 March 2013 1,385 1 View
I have a custom antibody (it works for Western blot and immunohistochemistry) that I would like to try for immunoprecipitation. I know that the antibody successfully binds my beads (I'm...
03 March 2013 4,856 7 View
Does anyone know of a chemical lysis method for total protein isolation from coelomocytes? I'm currently using physical disruption in the presence of detergent. This gets me a fair amount of my...
01 January 2013 4,289 5 View
I'm designing some primers for qRTPCR. Does anyone have any suggestions for a good qRTPCR primer design tool? (I expect to need to check for hairpins and dimers and what not, but would prefer...
09 September 2012 1,464 5 View
We are studying cell proliferation in a cell type (from a marine invertebrate) that is non-adherent. Does anyone have suggestions for method(s) to evaluate EDU labeling in non adherent cells? We...
09 September 2012 3,765 17 View
I'm looking to buy gels for the second dimension of IEF, and am attracted to the Bio-Rad Criterion XT gels - largely for their relatively long shelf-life (either the Bis-Tris with MOPS running...
08 August 2012 3,636 12 View
I'm somewhat new to IP and am looking for a good kit (or bead type) to isolate my proteins (large family of related proteins; we have an antibody specific for a conserved region). My antibody is a...
08 August 2012 5,091 5 View
We are studying cell proliferation in a cell type (from a marine invertebrate) that is non-adherent. Does anyone have suggestions for method(s) to evaluate EDU labeling in non adherent cells?...
08 August 2012 658 1 View
I'm doing 2-dimensional gel electrophoresis and want to run two samples next to my strip for comparison: marker (so check size) and the rehydrated protein (for comparison to the 2D spots). I know...
06 June 2012 3,456 14 View
I am isolating a protein by metal affinity chromatography, concentrating and desalting it, and then drying it by vacuum centrifugation. I would like to be able to quantify my protein at various...
06 June 2012 10,106 61 View
I'm making a stock of O'Farrell solution for SDS-PAGE gel casting. The recipe calls for 1.5M Tris pH 8.8 and 0.4% SDS in water, final pH 8.8. Each time I or a lab-mate try making this solution,...
05 May 2012 2,019 10 View
I have been using ampholytes pH 3-10 during isoelectric focusing of pH 3-10 strips. I'm now only looking at the basic range and am wondering if I can use these ampholytes with the pH 7-10 strips...
05 May 2012 4,022 9 View
I have a protein family with a histidine rich region that I am successfully isolating by metal affinity chromatography. I have previously run these samples on one-dimensional SDS-PAGE gels...
05 May 2012 3,190 33 View
