I'm looking to buy gels for the second dimension of IEF, and am attracted to the Bio-Rad Criterion XT gels - largely for their relatively long shelf-life (either the Bis-Tris with MOPS running buffer or Tris-acetate with Tricine running buffer).
Can these gels be run with traditional Tris/Glycine/SDS running buffer, or some other home-made buffer, instead of the Bio-Rad buffers?
Well I used them a while ago with a MOPS home-made running buffer and it worked fine. Also used them with SDS home-made buffer and never had any problem. Some of my previous co-workers still use the same gels with home-made buffers and never had a problem, as far as I know.
Hope it helps you :)
I am not sure if the gels were these exact ones, but we do run them with homemade running buffer Tris-GLycine/SDS
Yes, you can run them with homemade running buffer. There is no problem.
Great, thanks so much! Just wanted to be sure I wouldn't have to rely on buying buffer if I switch to these gels.
Thanks!
Bio-Rad is pretty bad about implying that you need to use their kits when ever you want to use any of their consumables. Rest assured that in most cases it is not necessary. Homemade buffers will work fine.
home made 1x Tris-glycine buffer works quite well for criterion XT gels.
I use all the time with home made buffer. I never had any problem! Please go for it!
No problem. home made Tris-glycine buffer works fine with criterion XT gels. Also works fine with Tris-SDS buffer
@Daniel, that's interesting to hear. Have you tried/heard anything about their Criterion TGX gels? Thats the another batch of gels I've been considering in place of the Tris-HCl gels I'm currently using
Could you elaborate on what you mean by "terrible results" compared to the Tris-HCl gels?
Daniel, are you performing "wet" tank transfers or using a semi-dry system?
As for buffers, you can always use the MES buffer receipe from the back of the Invitrogen NuPAGE manual. The gel formulations are the same and it works in our hands. But Tris/Glycine/SDS is always going to be the cheaper option.
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