I'm somewhat new to IP and am looking for a good kit (or bead type) to isolate my proteins (large family of related proteins; we have an antibody specific for a conserved region).
My antibody is a polyclonal IgG from rabbit. My proteins of interest range from 20kD-200kD in size (I don't know if that matters). The antibody has a decent number of primary amines.
I'm looking to elute my proteins without the antibody, and would prefer not to denature my proteins.
I don't have any experience with commercial IP kits, so any suggestions would be appreciated.
Thanks!