I am trying to amplify my gene of interest (GOI, 2000 kb long) by PCR. My previously designed primers are not working. How can I optimize the primers?

As I wanted to amplify my GOI, I chose first 18 nucleotides including start codon in my forward primer. I add BAMHI recognition site at the beginning and 3 nucleotide extension for the efficient...

12 January 2021 3,327 3 View

Does anyone knows the solubility or miscibility of xylene in tetrachloroethylene?

I need to learn if xylene and tetrachloroethylene are miscible or not. It is also will be usefull to learn a solvent that is not miscible with tetrachloroethylene. Thanks,

09 December 2020 3,383 2 View

I treated my fixated cells with PI for Cell Cycle Analysis but our Flow Cytometry did not work. How long can I keep my samples stable(+4) with PI dye?

I prepared my samples with ethanol, RNase and PI. However I could not use the Flow Cytometry machine because of an technical problem. I covered my samples with foil and put them in +4. As my...

21 November 2020 9,132 6 View

Do you have any practical methods of dipping the same amount of leads into the cell as a working electrode each time?

Dear colleagues, What kind of way do you follow to immerse the same amount of heads as pencil graphite electrodes into the cell every time for the cyclic voltammetry in sensor studies? May you...

18 October 2020 6,045 4 View

How to start with MFCC's features extraction for speaker identification ?

What are the next steps in speaker identification after we extract the MFCC features? Thank you so much.

09 May 2020 3,125 1 View

How should I cite the images (or should I according to my description below)?

Hello! I have a question regarding citing the images that I have draw for articles. I have planned the basic representation by Biorender for myself, I am happy with my design at this point. Now...

22 April 2019 8,717 5 View

What is the optimum dose for in vivo administration of fluorescent ag sio2 nanoparticles?

I'd like to administer Rhodamine B-AgSiO2 nanoparticles that I commercially purchased to mouse brain via carotid artery. The solution I have has the concentration of 0,25 mg/ml. What should be the...

03 April 2018 5,615 2 View

Which solvent(s) dissolves for both nylon 6 and SEBS g-MA?

I need one solvent or solvent system (max 2 solvent) which is suitable for my working polymers (nylon 6 and SEBS g-MA). If any one has an idea regarding this issue please share with me. Thank you.

03 October 2017 4,535 3 View

Is there a soil phosphate enrichment in the archaeological layers at palaeolithic open air sites?

At neolithic to modern sites it is often possible to spatially delimit a settlement or even classify functional areas with the help of a soil phosphate mapping. Does anyone know of such attempts...

27 September 2017 5,160 5 View

No title

01 February 2016 7,728 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View