Zeynep Hein

2 Questions 7 Answers 0 Followers

Questions related from Zeynep Hein

Can I use ECF subsrate in ELISA?

I wonder if it is possible to use ECF substrate which fluoresces when alkaline phosphatase is present. We have a fluorimeter (plate reader) so I thought of using our ECf to develop an ELISA...

05 September 2013 5,791 1 View

Does cycloheximide get old?

I am trying to block protein synthesis in living cells with using cycloheximide. I am monitoring this by labeling proteins with 35S-methionine during CHX treatment. I've tried several...

07 December 2012 8,260 3 View