I am trying to amplify my gene of interest (GOI, 2000 kb long) by PCR. My previously designed primers are not working. How can I optimize the primers?

As I wanted to amplify my GOI, I chose first 18 nucleotides including start codon in my forward primer. I add BAMHI recognition site at the beginning and 3 nucleotide extension for the efficient...

12 January 2021 3,327 3 View

Does anyone knows the solubility or miscibility of xylene in tetrachloroethylene?

I need to learn if xylene and tetrachloroethylene are miscible or not. It is also will be usefull to learn a solvent that is not miscible with tetrachloroethylene. Thanks,

09 December 2020 3,383 2 View

I treated my fixated cells with PI for Cell Cycle Analysis but our Flow Cytometry did not work. How long can I keep my samples stable(+4) with PI dye?

I prepared my samples with ethanol, RNase and PI. However I could not use the Flow Cytometry machine because of an technical problem. I covered my samples with foil and put them in +4. As my...

21 November 2020 9,132 6 View

Do you have any practical methods of dipping the same amount of leads into the cell as a working electrode each time?

Dear colleagues, What kind of way do you follow to immerse the same amount of heads as pencil graphite electrodes into the cell every time for the cyclic voltammetry in sensor studies? May you...

18 October 2020 6,045 4 View

How to start with MFCC's features extraction for speaker identification ?

What are the next steps in speaker identification after we extract the MFCC features? Thank you so much.

09 May 2020 3,125 1 View

How should I cite the images (or should I according to my description below)?

Hello! I have a question regarding citing the images that I have draw for articles. I have planned the basic representation by Biorender for myself, I am happy with my design at this point. Now...

22 April 2019 8,717 5 View

What is the optimum dose for in vivo administration of fluorescent ag sio2 nanoparticles?

I'd like to administer Rhodamine B-AgSiO2 nanoparticles that I commercially purchased to mouse brain via carotid artery. The solution I have has the concentration of 0,25 mg/ml. What should be the...

03 April 2018 5,615 2 View

Which solvent(s) dissolves for both nylon 6 and SEBS g-MA?

I need one solvent or solvent system (max 2 solvent) which is suitable for my working polymers (nylon 6 and SEBS g-MA). If any one has an idea regarding this issue please share with me. Thank you.

03 October 2017 4,535 3 View

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01 February 2016 7,728 2 View

How can I eliminate ethanol contamination from phage DNA samples?

I isolated M13 phage DNA from bacteria cells with NaI and ethanol steps (for sequencing of phage DNA), but I got very low values of 260/230 as 0,2. I think I have ethanol contamination in my...

03 May 2015 6,707 8 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Could the "incoming radiation (net radiation)" values be negative?

Could the "incoming solar radiation" values be negative? What is the difference between "global solar radiation" and "incoming solar radiation"? Which one might be measured negative values?

21 February 2021 7,726 9 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

How to determine the drug loading and the drug release capacity of a polymer micelle using UV spectroscopy?

Currently, I am working on PLA-PEG copolymer micelle for the drug delivery system. I have seen several papers where some researcher talks to centrifuge the drug-loaded micelle to separate the...

13 February 2021 5,822 7 View

How to calculate ultraviolet dose in Gy or J/kg?

Currently I'm doing a literature review regarding the comparisons of different types of radiation such as uv, electron beam and gamma radiation. But I'm having a difficult time converting uv unit...

11 February 2021 7,642 3 View

What is the UV-vis spectroscopy and HPLC UV detector?

I did spectrum scan to levothyroxine sodium which show highest absorption at wave length=230nm which the absorption =0.136 and it's absorption at 225nm =0.047 but when Injecting it on HPLC...

09 February 2021 1,631 3 View

What is the maximum wavelength and absorbance for dodecyl trimethylammonium bromide?

I am measuring the absorption of dodecyl trimethylammonium bromide surfactant using UV spectrometer. I am trying to set the wave length range for the experiment. Please does anyone have a idea of...

07 February 2021 7,720 1 View

Are you interested to submit a paper in the special issue "Solar Radiation" in "Remote Sensing" ?

I you are interested to submit a paper in the special issue "Solar Radiation" in "Remote Sensing", please have a look on the 2 following files and/or on the web site :...

01 February 2021 2,954 3 View

How to find degree of deacetylation of chitosan colorimetrically?

I am working with low DD% chitosan. It doesn't dissolve completely in acidic solutions. How to calculate the degree of deacetylation by potentiometric and acid-base titration, UV spectroscopic...

27 January 2021 8,532 2 View