I am trying to amplify my gene of interest (GOI, 2000 kb long) by PCR. My previously designed primers are not working. How can I optimize the primers?

As I wanted to amplify my GOI, I chose first 18 nucleotides including start codon in my forward primer. I add BAMHI recognition site at the beginning and 3 nucleotide extension for the efficient...

12 January 2021 3,327 3 View

Does anyone knows the solubility or miscibility of xylene in tetrachloroethylene?

I need to learn if xylene and tetrachloroethylene are miscible or not. It is also will be usefull to learn a solvent that is not miscible with tetrachloroethylene. Thanks,

09 December 2020 3,383 2 View

I treated my fixated cells with PI for Cell Cycle Analysis but our Flow Cytometry did not work. How long can I keep my samples stable(+4) with PI dye?

I prepared my samples with ethanol, RNase and PI. However I could not use the Flow Cytometry machine because of an technical problem. I covered my samples with foil and put them in +4. As my...

21 November 2020 9,132 6 View

Do you have any practical methods of dipping the same amount of leads into the cell as a working electrode each time?

Dear colleagues, What kind of way do you follow to immerse the same amount of heads as pencil graphite electrodes into the cell every time for the cyclic voltammetry in sensor studies? May you...

18 October 2020 6,045 4 View

How to start with MFCC's features extraction for speaker identification ?

What are the next steps in speaker identification after we extract the MFCC features? Thank you so much.

09 May 2020 3,125 1 View

How should I cite the images (or should I according to my description below)?

Hello! I have a question regarding citing the images that I have draw for articles. I have planned the basic representation by Biorender for myself, I am happy with my design at this point. Now...

22 April 2019 8,717 5 View

What is the optimum dose for in vivo administration of fluorescent ag sio2 nanoparticles?

I'd like to administer Rhodamine B-AgSiO2 nanoparticles that I commercially purchased to mouse brain via carotid artery. The solution I have has the concentration of 0,25 mg/ml. What should be the...

03 April 2018 5,615 2 View

Which solvent(s) dissolves for both nylon 6 and SEBS g-MA?

I need one solvent or solvent system (max 2 solvent) which is suitable for my working polymers (nylon 6 and SEBS g-MA). If any one has an idea regarding this issue please share with me. Thank you.

03 October 2017 4,535 3 View

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01 February 2016 7,728 2 View

How can I eliminate ethanol contamination from phage DNA samples?

I isolated M13 phage DNA from bacteria cells with NaI and ethanol steps (for sequencing of phage DNA), but I got very low values of 260/230 as 0,2. I think I have ethanol contamination in my...

03 May 2015 6,707 8 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

How to define the sink temperature and film coefficient?

Hi, everyone. I am considering the heat conviction during compacting simulation by Abaqus. I was wondering that how to define the sink temperature and film coefficient? Is there any references...

21 February 2021 6,197 1 View

No title

The regenerated cellulose film was prepared by zinc chloride/H2O system. The zinc chloride had been washed away in the regeneration process, and the wet film which was not dried had uniform...

18 February 2021 9,538 1 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

How to visualize stress in a coating defined by "Skin" in Abaqus?

Hello All! I'm trying to model a contact between a coated substrate and a tip in Abaqus. The coating is ultra-thin compared to the geometry of the substrate. So I defined the coating using the...

16 February 2021 1,594 3 View