I am trying to amplify my gene of interest (GOI, 2000 kb long) by PCR. My previously designed primers are not working. How can I optimize the primers?

As I wanted to amplify my GOI, I chose first 18 nucleotides including start codon in my forward primer. I add BAMHI recognition site at the beginning and 3 nucleotide extension for the efficient...

12 January 2021 3,327 3 View

Does anyone knows the solubility or miscibility of xylene in tetrachloroethylene?

I need to learn if xylene and tetrachloroethylene are miscible or not. It is also will be usefull to learn a solvent that is not miscible with tetrachloroethylene. Thanks,

09 December 2020 3,383 2 View

I treated my fixated cells with PI for Cell Cycle Analysis but our Flow Cytometry did not work. How long can I keep my samples stable(+4) with PI dye?

I prepared my samples with ethanol, RNase and PI. However I could not use the Flow Cytometry machine because of an technical problem. I covered my samples with foil and put them in +4. As my...

21 November 2020 9,132 6 View

Do you have any practical methods of dipping the same amount of leads into the cell as a working electrode each time?

Dear colleagues, What kind of way do you follow to immerse the same amount of heads as pencil graphite electrodes into the cell every time for the cyclic voltammetry in sensor studies? May you...

18 October 2020 6,045 4 View

How to start with MFCC's features extraction for speaker identification ?

What are the next steps in speaker identification after we extract the MFCC features? Thank you so much.

09 May 2020 3,125 1 View

How should I cite the images (or should I according to my description below)?

Hello! I have a question regarding citing the images that I have draw for articles. I have planned the basic representation by Biorender for myself, I am happy with my design at this point. Now...

22 April 2019 8,717 5 View

What is the optimum dose for in vivo administration of fluorescent ag sio2 nanoparticles?

I'd like to administer Rhodamine B-AgSiO2 nanoparticles that I commercially purchased to mouse brain via carotid artery. The solution I have has the concentration of 0,25 mg/ml. What should be the...

03 April 2018 5,615 2 View

Which solvent(s) dissolves for both nylon 6 and SEBS g-MA?

I need one solvent or solvent system (max 2 solvent) which is suitable for my working polymers (nylon 6 and SEBS g-MA). If any one has an idea regarding this issue please share with me. Thank you.

03 October 2017 4,535 3 View

No title

01 February 2016 7,728 2 View

How can I eliminate ethanol contamination from phage DNA samples?

I isolated M13 phage DNA from bacteria cells with NaI and ethanol steps (for sequencing of phage DNA), but I got very low values of 260/230 as 0,2. I think I have ethanol contamination in my...

03 May 2015 6,707 8 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View