Whats the Alternate for cell recovery solution from matrigel ??

Hello, my work involves growing cells in the matrigel and for recovering cells from matrigel i usually use cell recovery solution from corning. As now i am curious to know if theres an alternate...

20 November 2020 1,148 3 View

Why do mammalian cells in culture undergo necrosis?

I am observing a large necrotic population in glioblastoma cells that I am culturing in 10% DMEM medium. In spite of changing all parameters of culturing and making sure of the sterilitu, the...

05 November 2020 9,280 3 View

How to purify polymer-peptide complex?

I am trying to conjugate a 12 amino acid peptide (MW 1565Da) to PLGA-PEG-maleimide copolymer (MW: 35097Da). The conjugation reaction takes place in ACN: DMF as a solvent. Can someone please...

19 October 2020 636 5 View

Modeling of benzene recovery process by extractive distillation?

I am trying to model a Benzene recovery process by extractive distillation in Aspen plus. The calculation type chosen for the radfrac column is 'equilibrium' with the tray efficiency of 75%. i...

04 February 2020 9,279 3 View

Can i grow hacat and L929 cells with dmem high glucose + 4mM glutamine?

I am gonna grow hacat and L929 cells and can i grow these cell line with dmem high glucose+ 4mM L glutamine? Which is more preferable 2mM or 4mM glutamine? Thanks in advance.

09 March 2019 4,028 3 View

Why do MCF7 spheroids not get formed using the low-adhesion well-plate technique?

I have been trying to culture MCF7 spheroids by seeding 5000 cells in DMEM, in a low-adhesion 96 well-plate (Corning) and then centrifuging at 1000g for 10 mins. However, I am only able to get...

06 March 2017 3,510 4 View

What is the best protocol for subcellular fractionation of Gram-positive bacteria?

11 January 2017 9,996 1 View

Can we add tris buffer powder while preparing dehydrated culture media as buffering agent?

Can we add tris buffer powder while preparing dehydrated culture media as buffering agent? or we have to prepare the solution only?

30 May 2016 3,591 3 View

How to increase the metallic sheen in EMB agar?

how to increase the metallic sheen of E.coli on EMB agar?

09 May 2016 3,616 4 View

Any idea on what might hamper the opacity of Baird Parker agar plate due to addition of Egg Yolk Emulsion?

Any idea on opacity of baird parker agar plate after addition of egg yolk emulsion? In my agar plate after addition of egg yolk emulsion opacity is less, what can be the reason or possibility for...

13 April 2016 3,812 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

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28 February 2021 9,936 2 View