Zohaib Ul Hassan

3 Questions 1 Answers 0 Followers

Questions related from Zohaib Ul Hassan

Protocol for performing Overlap-Extension PCR? How can I overlap two fragments approx. 6kb in size with an overlaping region of almost 650 bp?

I want to amplify the approx. 12kb fragment by overlap extension of two PCR products of 6kb in size. - What protocol should I adopt for the Overlap_Extension PCR? - Which Polymerase gives better...

30 June 2020 5,333 0 View

Can I use a single Illumina iseq100 cartridge for two different sets (kinds) of libraries simultaneously?

I want to sequence the amplicons with two different library prep kits. 1. Nextera DNA Flex Library Prep. kit 2. QIAseq Ultralow Input Library Kit Can I use the libraries from both preparation...

01 June 2020 1,841 3 View

What is a method for understanding the molecular mechanism of protein X?

You carried out a Genetic Screen for genes involved in the stress response in E. coli. From this screen you identified a gene that encodes a 50 kDa flexible protein (Protein X), which does not...

16 September 2017 1,370 0 View