I want to do the DNA fragmentation in sperm on next day , after transfer it from one city to another city which is having facilities. kindly suggest.
thanks,
you must first fix the cells using a methanol/acetic acid mixture (3 volumes/1 volume) for 20 min
I've been working with DFT-VASP and have a few questions regarding the calculation of screened/unscreened plasma frequency. I'd like to outline my workflow and the parameters I've used, and I'd...
03 November 2023 9,915 1 View
Steps to Calculate Transport Properties for Metallic Systems with BoltzTraP2 and VASP I'm currently working on calculating transport properties, specifically carrier concentration and...
31 October 2023 4,535 0 View
I am planning to perform the functional study of gene whuch is supplied maternally, so morpholino-mediated knockdown assay won't work. Also about the CRISPR mediated knockout study need to wait...
27 December 2020 889 1 View
I was trying to mimic the deflection of a cantilever beam in wire on to a model with a portion of the beam replaced by a spring which provides a bending stiffness. I gave a usual spring with...
01 July 2020 7,142 4 View
I have been trying to add the joint axial stiffness and bending stiffness into the model in ABAQUS (please check the attached image, the nodes are the joint). I have been trying to add these...
01 March 2020 5,229 3 View
identification of specific combiners in maize are identified using any of the mating designs like LineXTester or Diallele mating system...but why are they not used in the commercial hybrid...
23 February 2020 1,043 3 View
I am currently modelling a joint of a geodesic dome. I previously ran an analysis only with preload of bolts ignoring all contact surfaces of the shank of the bolt and other geometry . This time...
03 June 2019 5,037 5 View
We are doing a project on adsorption of hexavalent chromium using agricultural wastes. I would want to generate adsorption isotherms for our data. Would I be able to know the procedure for the...
24 May 2019 1,810 13 View
I am going to do a design project, for that I have planned to design some useful environmental structure other than water and waste water treatment plants. We searched for biomedical waste water...
24 June 2018 228 9 View
I am a final year UG student, this semester we need to do design project. But we have learnt only member design in our curriculum, so I am looking for a structure by designing that we can get a...
15 June 2018 2,117 7 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Census data seems only to reach the year 2011, but there might be estimates somewhere that I cannot find? I am looking not only for the largest cities but also towns etc. Thanks!
10 August 2024 857 4 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
I am trying to analyse data from a survey examining what variables affect teachers perceived barriers to incorporating technology into their classroom. I have 5 predictor variables however my DV...
06 August 2024 1,752 3 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
We assume this to be true. We also assume that the vacuum bomb is the latest version of explosives with an explosive power of a few to ten kg of TNT equivalent. It has the unique characteristic of...
04 August 2024 4,534 1 View