I stored my fixed cells ( fixed with pfa) in FACS buffer (2% FBS in PBS) at minus 20 celcius, would it be okay?
You could check your cells under the microscope and compare the scatter pattern in FACS with freshly fixed cells.
I would not freeze them, put them at 4 degrees
Even though they are fixed, your cells won't cryopreserve well. Try adding 10% DMSO to your buffer to prevent damage or use 10% DMSO in FBS to store your cells (PMID:27798817).
You can keep fixed cells in FACS buffer at 4°C for few days.
I saw this colony which looks like cells that probably growing upward, is it by any chance an iPSC colony? I know iPSC colony normally would not looks like this, but has anyone encounter...
26 December 2022 4,079 4 View
I tried to reprogram MSC to iPSC and found my culture cloudy this morning, just want to ask whether this a contamination or not. thanks
13 December 2022 8,630 4 View
I've been measuring the gas emission of a lab-scale peatland experiment with a electrochemical sensor. The output of the sensor measures the concentration in ppm. I want to change this data to EF....
25 May 2021 6,977 1 View
I'm just wondering if that is possible to simulate microchannel flow behavior in a larger channel with dimensionless parameter scaling of the operating condition
03 January 2021 7,873 1 View
I want to calibrate my building energy simulation from eQuest using the measured utility bills data (electricity and gas). I have three years of bills data (2016-2018), which bill data should I...
26 July 2019 9,565 2 View
My plasmid DNA sample is shown in white box on the picture below. It is almost unseen. Meanwhile, another bands is very clear to be seen altough their plasmid DNA concentration is lower than my...
26 February 2016 6,731 8 View
We tried to reprogram MSC. After 29 days post-electroporation of episomal vector (carrying Yamanaka Factors), we found this on our culture (see pictures). Is it iPSC? we planned to terminate it if...
01 January 1970 9,031 5 View
7 days ago I transfected my MSC with episomes carrying reprogramming factors and today I found this structure (see the pictures) which seems like it consists of small cells (?), do you guys think...
01 January 1970 4,381 1 View
Hello experts in genetic modification, according to your experiences, do 2 mm and 4 mm electroporation cuvette have any differences in performance? or they just different in volume thank you
01 January 1970 8,067 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!
08 August 2024 6,242 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...
07 August 2024 2,569 1 View
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,013 4 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View