7 days ago I transfected my MSC with episomes carrying reprogramming factors and today I found this structure (see the pictures) which seems like it consists of small cells (?), do you guys think this is iPSC colony?
I never actually have experienced a successful reprogramming before but I already got my hand on iPSC culture and to my knowledge iPSC Colony doesn't look like this.
Let me know your thought
have a good day everyone
Solange Martins
Usually iPSC colonies start to appear after two weeks in culture, and I have never seen anything like that in my reprogrammings. The colonies are usually round or elongated and have well defined borders.
Try to wait a bit more, and watch out for contamination.
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